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anti srf
Anti Srf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+srf/pm41861925-89-9-11
Average 86 stars, based on 1 article reviews
Anti Srf, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+srf/pm41861925-89-9-11
Average 86 stars, based on 1 article reviews
anti srf - by Bioz Stars,
2026-10
86/100 stars
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Immunoprecipitation:Article Title: Zinc finger transcription factor Egf1 promotes non-alcoholic fatty liver disease Article Snippet: .. Aliquots of lysates containing 100 lg of protein were used for each immunoprecipitation reaction with Binding Assay:Article Title: Zinc finger transcription factor Egf1 promotes non-alcoholic fatty liver disease Article Snippet: .. Aliquots of lysates containing 100 lg of protein were used for each immunoprecipitation reaction with Adsorption:Article Title: Zinc finger transcription factor Egf1 promotes non-alcoholic fatty liver disease Article Snippet: .. Aliquots of lysates containing 100 lg of protein were used for each immunoprecipitation reaction with Incubation:Article Title: SRF fusion oncogenes encode constitutively activated chimeric transcription factors in myoid soft tissue tumors Article Snippet: Samples (30 μg) were mixed with 4x Laemmli buffer (0.2 M Tris-HCl pH 6.8, 8% SDS, 0.4% bromophenol blue, 40% glycerol, 2.8% β-mercaptoethanol), boiled for 5 min, and analyzed by western blot using Novex Tris-Glycine 4–12% gels (Thermo Fisher Scientific) and PVDF membranes (Amersham Hybond P, GE Healthcare). .. Membranes were blocked in 5% milk for 1 h at room temperature, then incubated overnight at 4°C with the indicated primary antibodies : anti-HA-tag (#3724, Cell Signaling Technology, Danvers, MA, USA), anti-Myc-Tag (#2272, Cell Signaling Technology), anti-phospho-STAT1 (#9167, Cell Signaling Technology), anti-STAT1 (#610115, BD BioSciences), anti-phospho-STAT3 (#9145, Cell Signaling Technology), anti-STAT3 (#12640, Cell Signaling Technology), anti-phospho-STAT5 (#9351, Cell Signaling Technology), anti-STAT5 (#94205, Cell Signaling Technology), Article Title: circFAM53B-1 and circFAM53B-2 drive VSMC phenotypic modulation via ISG15-mediated suppression of SRF. Article Snippet: Although circRNAs have been widely studied in various disease contexts, their roles in vascular smooth muscle cells (VSMCs) phenotypic modulation remain incompletely understood.. This study aims to investigate the regulatory functions and molecular mechanisms of two newly identified circRNAs, circFAM53B-1 and circFAM53B2, in VSMCs phenotypic switching.. Here, we show that both circFAM53B-1 and circFAM53B-2 were upregulated by PDGF-BB in a KLF4-dependent manner. Article Title: Single-molecule analysis reveals that IPMK enhances the DNA-binding activity of the transcription factor SRF. Article Snippet: For western blotting analysis, TMR-positive cells were sorted with FACS (Bio-Rad S3e). .. For chromatin immunoprecipitation (ChIP) assay, magnetic beads were washed with radioimmunoprecipitation assay (RIPA) wash buffer and incubated with either normal rabbit IgG (Cell Signaling Technology, 2729) or Article Title: Single-molecule analysis reveals that IPMK enhances the DNA-binding activity of the transcription factor SRF Article Snippet: For western blotting analysis, TMR-positive cells were sorted with FACS (Bio-Rad S3e). .. For chromatin immunoprecipitation (ChIP) assay, magnetic beads were washed with radioimmunoprecipitation assay (RIPA) wash buffer and incubated with either normal rabbit IgG (Cell Signaling Technology, 2729) or Staining:Article Title: circFAM53B-1 and circFAM53B-2 drive VSMC phenotypic modulation via ISG15-mediated suppression of SRF. Article Snippet: Although circRNAs have been widely studied in various disease contexts, their roles in vascular smooth muscle cells (VSMCs) phenotypic modulation remain incompletely understood.. This study aims to investigate the regulatory functions and molecular mechanisms of two newly identified circRNAs, circFAM53B-1 and circFAM53B2, in VSMCs phenotypic switching.. Here, we show that both circFAM53B-1 and circFAM53B-2 were upregulated by PDGF-BB in a KLF4-dependent manner. Chromatin Immunoprecipitation:Article Title: Single-molecule analysis reveals that IPMK enhances the DNA-binding activity of the transcription factor SRF. Article Snippet: For western blotting analysis, TMR-positive cells were sorted with FACS (Bio-Rad S3e). .. For chromatin immunoprecipitation (ChIP) assay, magnetic beads were washed with radioimmunoprecipitation assay (RIPA) wash buffer and incubated with either normal rabbit IgG (Cell Signaling Technology, 2729) or Article Title: Single-molecule analysis reveals that IPMK enhances the DNA-binding activity of the transcription factor SRF Article Snippet: For western blotting analysis, TMR-positive cells were sorted with FACS (Bio-Rad S3e). .. For chromatin immunoprecipitation (ChIP) assay, magnetic beads were washed with radioimmunoprecipitation assay (RIPA) wash buffer and incubated with either normal rabbit IgG (Cell Signaling Technology, 2729) or Magnetic Beads:Article Title: Single-molecule analysis reveals that IPMK enhances the DNA-binding activity of the transcription factor SRF. Article Snippet: For western blotting analysis, TMR-positive cells were sorted with FACS (Bio-Rad S3e). .. For chromatin immunoprecipitation (ChIP) assay, magnetic beads were washed with radioimmunoprecipitation assay (RIPA) wash buffer and incubated with either normal rabbit IgG (Cell Signaling Technology, 2729) or Article Title: Single-molecule analysis reveals that IPMK enhances the DNA-binding activity of the transcription factor SRF Article Snippet: For western blotting analysis, TMR-positive cells were sorted with FACS (Bio-Rad S3e). .. For chromatin immunoprecipitation (ChIP) assay, magnetic beads were washed with radioimmunoprecipitation assay (RIPA) wash buffer and incubated with either normal rabbit IgG (Cell Signaling Technology, 2729) or Radio Immunoprecipitation:Article Title: Single-molecule analysis reveals that IPMK enhances the DNA-binding activity of the transcription factor SRF. Article Snippet: For western blotting analysis, TMR-positive cells were sorted with FACS (Bio-Rad S3e). .. For chromatin immunoprecipitation (ChIP) assay, magnetic beads were washed with radioimmunoprecipitation assay (RIPA) wash buffer and incubated with either normal rabbit IgG (Cell Signaling Technology, 2729) or Article Title: Single-molecule analysis reveals that IPMK enhances the DNA-binding activity of the transcription factor SRF Article Snippet: For western blotting analysis, TMR-positive cells were sorted with FACS (Bio-Rad S3e). .. For chromatin immunoprecipitation (ChIP) assay, magnetic beads were washed with radioimmunoprecipitation assay (RIPA) wash buffer and incubated with either normal rabbit IgG (Cell Signaling Technology, 2729) or Real-time Polymerase Chain Reaction:Article Title: Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity. Article Snippet: Oncogenic protein dosage is tightly regulated to enable cancer formation but how this is regulated by translational control remains unknown.. The Myc oncogene is a paradigm of an exquisitely regulated oncogene and a driver of pancreatic ductal adenocarcinoma (PDAC).. Here we use a CRISPR interference screen in PDAC cells to identify activators of selective MYC translation. Isolation:Article Title: Functional screen identifies RBM42 as a mediator of oncogenic mRNA translation specificity. Article Snippet: Oncogenic protein dosage is tightly regulated to enable cancer formation but how this is regulated by translational control remains unknown.. The Myc oncogene is a paradigm of an exquisitely regulated oncogene and a driver of pancreatic ductal adenocarcinoma (PDAC).. Here we use a CRISPR interference screen in PDAC cells to identify activators of selective MYC translation. |
![Contractile Gene Expression Is Downregulated in Chol-Loaded hVSMCs (A, B) Human vascular smooth muscle cells (hVSMCs) were treated with cholesterol (Chol) (5 μg/mL) or 0.2% bovine serum albumin (control [CT]) for 24 hours and 48 hours and gene expression <t>of</t> <t>Acta2</t> , Tagln , Cnn1, Myocd, and <t>Srf</t> were determined by quantitative polymerase chain reaction. (C) hVSMCs were treated with Chol (5 μg/mL) or 0.2% bovine serum albumin (CT) for 24 hours and protein expression of α–smooth muscle actin (α-SMA) and CNN1 were determined by Western blotting (representative blots shown). Densitometry showing the (D) α-SMA and (E) CNN1 band intensities normalized to GAPDH. For data analysis, unpaired Student’s t -testing was performed for comparing the means of 2 groups. For 2 or more independent groups, 1-way analysis of variance followed by Dunnett post hoc test was performed. A P value of ≤0.05 was considered significant. Data are presented as the mean ± SEM of 3 independent experiments, and P values are as indicated (∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001).](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_5216/pmc12925216/pmc12925216__gr1.jpg)
